• Catalog Number:

  E1A7148A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291(P-R-TP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1

• Specificity Sensitivity:

  MEK1 (phospho-Thr291) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 291.

• References:

  Kevin D. Burroughs, et,al. (2003) Mol. Cancer Res; 1: 312. Michael J. Piatelli, et,al. (2002) J. Biol. Chem; 277: 12144-12150. Margaret M. Morgan, et,al. (2001) J. Immunol; 167: 5708. Herbert Schramek, et,al. (2003) Am J Physiol Cell Physiol; 285: C652-C661.

• All Sites:

  Human: Thr 291; Mouse: Thr291; Rat: Thr291

Western blot analysis of extracts from K562/A431 cells untreated or treated with Serum (10%, 30mins), using MEK1 (Ab-291) antibody (Line 1 and 2) and MEK1 (phospho-Thr291) antibody (Line 3 and 4).