• Catalog Number:

  E1A7146A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine Ser218/222(I-D-SP-M-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Other Names:

  Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1

• Specificity Sensitivity:

  MEK1/2(Phospho-Ser218/222) antibody detects endogenous levels of MEK1(Phospho-Ser218) and MEK2(Phospho-Ser222).

• References:

  Seger R., J. Biol. Chem. 267:25628-25631(1992). Zheng C.-F., J. Biol. Chem. 268:11435-11439(1993). Zheng C.-F., EMBO J. 13:1123-1131(1994). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser218/Ser222; Mouse: Ser218/Ser222; Rat: Ser218/Ser222

Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using MEK1/2(Phospho-Ser218/222) antibody.