• Catalog Number:

  E1A7145A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of threonine 319 (V-T-TP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02078

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

• Specificity Sensitivity:

  MEF2A (phospho-Thr319) antibody detects endogenous levels of MEF2A only when phosphorylated at threonine 319.

• References:

  Rafael Toro, J. Biol. Chem., Dec 2004; 279: 52087 - 52094. Silvia Mora and Jeffrey E. Pessin, J. Biol. Chem., May 2000; 275: 16323. Cyril Rauch and Paul T. Loughna, Am J Physiol Cell Physiol, Mar 2005; 288: C593 - C605. Ming Zhao, Mol. Cell. Biol., Jan 1999; 19: 21 - 30.

• All Sites:

  Human: Thr319; Mouse: Thr317; Rat: Thr311

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (phospho-Thr319) antibody.

Western blot analysis of extracts from K562 cells treated with UV (15mins), using MEF2A (phospho-Thr319) antibody.