Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29966
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Alter Name:
80K-L protein, MACS, MARCS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 81 kDa protein, light chain
Specificity Sensitivity:
MARCKS (phospho-Ser162) antibody detects endogenous levels of MARCKS only when phosphorylated at serine 162.
References:
Dongjun Li, J. Neurosci., Feb 2005; 25: 1914 - 1923.
Gamal Badr, J. Immunol., Jul 2005; 175: 302 - 310.
Magdalena J. Lorenowicz, Arterioscler. Thromb. Vasc. Biol., May 2007; 27: 1014 - 1022.
All Sites:
Human: Ser162; Mouse: Ser155; Rat: Ser155
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using MARCKS (phospho-Ser162) antibody.