• Catalog Number:

  E1A7142A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P29966

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  80K-L protein; MACS; MARCS; myristoylated alanine-rich C-kinase substrate; PKCSL; PRKCSL; protein kinase C substrate, 80 kDa protein, light chain

• Specificity Sensitivity:

  MARCKS (Phospho-Ser158) antibody detects endogenous levels of MARCKS only when phosphorylated at serine 158.

• References:

  Harlan D.M., J. Biol. Chem. 266:14399-14405(1991). Sakai K., Genomics 14-175-178(1992). Mungall A.J., Nature 425:805-811(2003).

• All Sites:

  Human: Ser159; Mouse: Ser152; Rat: Ser152

Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 30mins), using MARCKS (Phospho-Ser158) antibody.