• Catalog Number:

  E1A7139A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 393 (N-E-YP-T-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06239

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  EC 2.7.10.2; kinase Lck; LCK; LSK; LSK-T; lymphocyte-specific protein tyrosine kinase; P56-LCK; T cell-specific protein-tyrosine kinase

• Specificity Sensitivity:

  Lck (Phospho-Tyr393) antibody detects endogenous levels of Lck only when phosphorylated at tyrosine 393.

• References:

  Koga Y., Eur. J. Immunol. 16:1643-1646(1986). Perlmutter R.M., J. Cell. Biochem. 38:117-126(1988). Rouer E., Gene 84:105-113(1989).

• All Sites:

  Human: Ser24; Mouse: Ser24; Rat: Ser24

Peptide - + Western blot analysis of extracts from Jurkat cells, using Lck (Phospho-Tyr393) antibody. Immunofluorescence analysis of HeLa cells, using Lck (Phospho-Tyr393) antibody.