Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human JunB around the phosphorylation site of Serine 79.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17275
Applications:
IHC: 1:50~1:100 ELISA: 1:20000
IP: Various Dilution
Specificity Sensitivity:
JunB (Phospho-Ser79) Antibody detects endogenous levels of JunB only when phosphorylated at Serine 79.
References:
Narayanan K, et al. (2004) J Biol Chem. 279(43): 44294-442302.
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using JunB(Phospho-Ser79) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.