• Catalog Number:

  E1A7132A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of Threonine 91.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05412

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  c-Jun (Phospho-Thr91) Antibody detects endogenous levels of c-Jun only when phosphorylated at Threonine 91.

• References:

  Binetruy B, et al. (1991) Nature. 351: 122-127. Smeal T, et al. (1991) Nature. 354:494-496. Derijard B, et al. (1994) Cell. 76:1025-1037. Kyriakis J M, et al. (1994) Nature. 369: 156-160.

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using c-Jun (Phospho-Thr91) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts using c-Jun (Phospho-Thr91) Antibody. Line1: The extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with UV.