• Catalog Number:

  E1A7128A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SAPK/JNK around the phosphorylation site of threonine 183 (M-M-TP-P-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human ,Mouse, Rat

• SwissProtNo:

  P45984; P53779

• Applications:

  IHC: 1:50~1:100 ELISA: 1:4000

• Alter Name:

  Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; JNK-55; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAP kinase p49 3F12

• Specificity Sensitivity:

  SAPK/JNK (Phospho-Thr183) antibody detects endogenous levels of SAPK/JNK only when phosphorylated at threonine 183.

• References:

  Zhonghong Guan, et al. (1999) J. Biol. Chem; 274: 36200. Stefan Krautwald, et al. J. Immunol., Jun 1998; 160: 5874. Jongkyu Choi, et al. (2004) nvest. Ophthalmol. Vis. Sci; 45: 2696-2704. Gerhard Fritz and Bernd KainaMol, (2006) Biol. Cell; 17: 851-861.

• All Sites:

  Human: Thr183; Mouse: Thr183; Rat:Thr183

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Phospho-Thr183) antibody.

Western blot analysis of extracts from Hela cells, untreated or treated with Anisomycin (200ng/ml, 10mins), using SAPK/JNK (Phospho-Thr183) antibody.