• Catalog Number:

  E1A7124A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of serine 32 and serine 36 (H-D-SP-G-L-D-SP-M-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25963

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1

• Specificity Sensitivity:

  IκB-α (Phospho-Ser32/Ser36) antibody detects endogenous levels of IκB-α only when phosphorylated at serine 32 and serine 36.

• References:

  Haskill S., Cell 65:1281-1289(1991). Jungnickel B., J. Exp. Med. 191:395-402(2000). Liu B., Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser32+Ser36; Mouse: Ser32+Ser36; Rat: Ser32+Ser36

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IκB-α (Phospho-Ser32/Ser36) antibody. Western blot analysis of extracts from COS-7 cells, using IκB-α (Phospho-Ser32/Ser36) antibody.

P-peptide - + Immunofluorescence analysis of LOVO cells, using IκB-α (Phospho-Ser32/Ser36) antibody.