• Catalog Number:

  E1A7121A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  Insulin receptor substrate-1; IRS-1; IRS1

• Specificity Sensitivity:

  IRS-1 (Phospho-Ser312) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

• References:

  Araki E., Diabetes 42:1041-1054(1993). Nishiyama M., Biochem. Biophys. Res. Commun. 183:280-285(1992). Smith L.K., Biochem. Biophys. Res. Commun. 196:767-772(1993).

• All Sites:

  Human: Ser24; Mouse: Ser24; Rat: Ser24

Peptide - + Western blot analysis of extracts from HT-29 cells, using IRS-1 (Phospho-Ser312) antibody. Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) antibody.