• Catalog Number:

  E1A7119A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Integrin β3 around the phosphorylation site of tyrosine 785 (I-T-YP-R-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P05106

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  CD61 antigen, GP3A, GPIIIa, ITB3, Integrin beta-3 precursor, Platelet membrane glycoprotein IIIa, integrin beta-3

• Specificity Sensitivity:

  Integrin β3 (phospho-Tyr785) antibody detects endogenous levels of Integrin β3 only when phosphorylated at tyrosine 785.

• References:

  Anirban Datta, J. Biol. Chem., Feb 2002; 277: 3943 - 3949. Alison L. Jenkins, J. Biol. Chem., May 1998; 273: 13878. Scott D. Blystone, J. Biol. Chem., Nov 1997; 272: 28757. Rita I. Kirk, J. Biol. Chem., Sep 2000; 275: 30901.

• All Sites:

  Human: Thr785; Mouse: Thr784

Western blot analysis of extracts from 293 cells treated with Insulin (0.01U/ml, 15mins), using, using Integrin β3 (phospho-Tyr785) antibody (Line 1 and 2).