• Catalog Number:

  E1A7110A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04792

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.

• References:

  Tremolada L, et al. (2005) Proteomics. 5(3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124. Benn SC, et al. (2002) Neuron. 36(1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSP27 (phospho-Ser15) antibody.

Western blot analysis of extracts from HeLa cells using HSP27 (Ab-15) antibody (Line1 and 2) and HSP27 (phospho-Ser15) antibody (Line 3 and 4).