• Catalog Number:

  E1A7109A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q00613

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  HSTF1, Heat shock factor protein 1

• Specificity Sensitivity:

  HSF1 (Phospho-Ser303) antibody detects endogenous levels of HSF1 only when phosphorylated at serine 303.

• References:

  Parvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol; 291: C931-C945. Fumika Shinozaki, et,al. (2006) J. Biol. Chem; 281: 16361-16369. Eiichi Takaki, et,al. (2006) J. Biol. Chem; 281: 4931-4937. Jan-Jong Hung, et,al. (1998) J. Biol. Chem; 273: 31924.

• All Sites:

  Human: Ser303

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Phospho- Ser303) antibody.

Western blot analysis of extracts from MCF cells untreated or treated with TNF-α (20ng/ml, 30mins), using HSF1 (Ab-303) antibody (Line 1 and 2), and HSF1 (Phospho-Ser303) antibody (Line 3 and 4).