• Catalog Number:

  E1A7108A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HNF4α around the phosphorylation site of serine 313 (L-R-SP-Q-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P41235

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  hepatocyte nuclear factor 4-alpha; HNF-4; HNF-4-alpha; HNF4; HNF4A; NR2A1; TCF14; transcription factor 14; transcription factor HNF-4

• Specificity Sensitivity:

  HNF4α (Phospho-Ser313) antibody detects endogenous levels of HNF4α only when phosphorylated at serine 313.

• References:

  Kritis A.A., Gene 173:275-280(1996). Drewes T., Mol. Cell. Biol. 16:925-931(1996). Yamagata K., Nature 384:458-460(1996).

• All Sites:

  Human: Ser313; Mouse: Ser313; Rat: Ser313

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using HNF4α (Phospho-Ser313) antibody. Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using HNF4α (Phospho-Ser313) antibody.

P-peptide - + Immunofluorescence analysis of LOVO cells, using HNF4α (Phospho-Ser313) antibody.