Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P68431
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Specificity Sensitivity:
Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.
References:
Dai J, et al. (2005) Genes Dev 19(4): 472-488.
Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (phospho-Ser10) antibody.
Western blot analysis of extracts from HeLa cells using Histone H3.1 (phospho-Ser10) antibody.