• Catalog Number:

  E1A7106A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P16104

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  H2a/x

• Specificity Sensitivity:

  Histone H2A.X (Phospho-Ser139) antibody detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.

• References:

  Alpna Tyagi, et al. (2005) Carcinogenesis; 26: 1978-1987. James P, et al. (2002) Nucleic Acids Res; 30: 3698-3705. Mariana Yaneva, et al. (2005) Nucleic Acids Res; 33: 5320-5330. Jérôme Govin, et al. (2004) Eur. J. Biochem; 271: 3459-3469

• All Sites:

  Human: Ser139; Mouse: Ser139

Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Histone H2A.X (Ab-139) antibody (Line 1 and 2) and Histone H2A.X (Phospho-Ser139) antibody (Line 3 and 4).