• Catalog Number:

  E1A7104A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1248 (P-E-YP-L-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04626

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  HER2 (phospho-Tyr1248) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine1248.

• References:

  Ramsauer VP, et al. (2003) J Biol Chem; 278(32): 30142-7. Jepson S, et al. (2002) Oncogene; 21(49): 7524-32. Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93. Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19(3): 645-56.

Western blot analysis of extracts from HepG2 cells, using HER2 (phospho-Tyr1248) antibody.

Western blot analysis of extracts from HepG2 cells, using HER2 (phospho-Tyr1248) antibody.

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using HER2 (phospho-Tyr1248) antibody.