• Catalog Number:

  E1A7101A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of Serine498 (T-Q-SP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UQL6

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  HDAC5 (Phospho-Ser498) Antibody detects endogenous levels of HDAC5 only when phosphorylated at Serine 498.

• References:

  Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019. McKinsey TA, et al. (2000) Nature. 408(6808): 106-111.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using HDAC5 (Phospho-Ser498) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis of the extracts from NIH/3T3 cells using HDAC5 (Ab-498) Antibody and HDAC5 (Phospho-Ser498) Antibody.