• Catalog Number:

  E1A7097A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GSK3α around the phosphorylation site of Serine 21.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49840

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  GSK3α(Phospho-Ser21) Antibody detects endogenous levels of GSK3α only when phosphorylated at Serine 21.

• References:

  Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.

Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using GSK3α (Phospho-Ser21) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of extracts from ovary cancer cells. Line1: Using GSK3α(Phospho-Ser21) Antibody; Line2: The same antibody preincubated with synthesized non-phospho- peptide; Line3: The same antibody preincubated with synthesized phosphopeptide.