• Catalog Number:

  E1A7092A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15976

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 IP: Various Dilution

• Specificity Sensitivity:

  GATA-1 (phospho-Ser142) antibody detects endogenous levels of GATA-1 only when phosphorylated at serine 142.

• References:

  Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. Trainor C D, et al. (1990) Nature. 343: 92-96. Nichols K E, et al. (2000) Nat Genet. 24: 266-270.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GATA-1 (phospho-Ser142) antibody.

Western blot analysis of extracts from K562 cells using GATA-1 (phospho-Ser142) antibody.