• Catalog Number:

  E1A7083A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 925 (K-V-YP-E-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Specificity Sensitivity:

  FAK (phospho-Tyr925) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 925.

• References:

  Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22. Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83. Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.

| HepG2 | 293 | Western blot analysis of extracts using FAK (phospho- Tyr925) antibody.