• Catalog Number:

  E1A7082A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 861 (H-I-YP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  FAK (phospho-Tyr861) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 861.

• References:

  Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15. Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80. Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60. Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.

Western blot analysis using FAK (Ab-861) antibody (Line 1 and 2) and FAK (phospho-Tyr861) antibody (Line 3, 4, 5 and 6).