• Catalog Number:

  E1A7079A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 566 (Y-K-TP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15311

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  Cytovillin, EZRI, VIL2, Villin 2, p81

• Specificity Sensitivity:

  Ezrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 566.

• References:

  Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91 Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95 Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90 Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9

• All Sites:

  Human: Thr566 or Thr567; Mouse:Thr566 or Thr567; Rat: Thr567

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30mins) using Ezrin (Ab-566) antibody (Line 1 and 2) and Ezrin (phospho-Tyr566) antibody (Line 3 and 4).