• Catalog Number:

  E1A7078A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 167 (L-A-SP-T-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P03372

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  Estrogen Receptor-α (phospho-Ser167) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine167.

• References:

  Shah YM, et al. (2005). Mol Endocrinol.19 (3): 732-748. Sun M, et al. (2001). Cancer Res.61 (16): 5985-5991. Campbell RA, et al. (2001). J Biol Chem.276 (13): 9817-9824. Chen D, et al. (2000). Mol Cell.6 1): 127-137.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (phospho-Ser167) antibody.

Western blot analysis of extracts from MCF7 cells treated with EGF, using Estrogen Receptor-α (phospho-Ser167) antibody.