• Catalog Number:

  E1A7073A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-TP-E-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27361

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase

• Specificity Sensitivity:

  p44/42 MAP Kinase (Phospho-Thr202) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202.

• References:

  D. Betty Lew, et al. (1999) Am. J. Respir. Cell Mol. Biol; 21: 111. George W. Small, (2003) et al.J. Pharmacol. Exp. Ther; 307: 861. Jingui Yu, et al. (2005) Anesth. Analg; 101: 315-321. Panettieri, et al. (1999) Am J Physiol Lung Cell Mol Physiol; 277: 479.

• All Sites:

  Human:Thr202; Mouse: Thr202; Rat:Thr202

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200 ng/ml, 30mins), using p44/42 MAP Kinase (Ab-202) antibody (Line 1 and 2) and p44/42 MAP Kinase (phospho-Thr202) antibody (Line 3 and 4).