• Catalog Number:

  E1A7065A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR

• Specificity Sensitivity:

  EGFR (Phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.

• References:

  Inoue A, et al. (2005) PLoS Med; 2(1): e13. Sun H, et al. (2004) EMBO J; 23(1): 100-110. Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720. Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674. O’Rourke DM, et al. (1997) Proc Natl Acad Sci USA; 94(7): 3250-3255. Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441.

• All Sites:

  Human: Tyr869; Mouse: Tyr871; Rat: Tyr870

Western blot analysis of extracts from A431 cells untreated or treated with EGF (40μM, 10mins), using EGFR (Ab-869) antibody (Line1 and 2) and EGFR (phospho-Tyr869) antibody (Line 3 and 4).