• Catalog Number:

  E1A7058A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q99704

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  DOK1 Docking protein 1

• Specificity Sensitivity:

  p62Dok (Phospho-Tyr398) antibody detects endogenous levels of p62Dok only when phosphorylated at tyrosine 398.

• References:

  Michael J. Wick, et,al. (2001) J. Biol. Chem; 276: 42843-42850. Paul D. Simoncic, et,al. (2006) Mol. Cell. Biol; 26: 4149-4160. Nadia Dubé, et,al. (2004) PNAS; 101: 1834. Adam C. Yopp, et,al . (2005) J. Immunol; 175: 2913-2924.

• All Sites:

  Human: Tyr398; Mouse: Tyr397; Rat: Tyr472

Western blot analysis of extracts from K562 cells, untreated or treated with Starvation (24 hours), using p62Dok (Ab-398) antibody (Line 1 and 2) and p62Dok (Phospho-Tyr398) antibody (Line 3 and 4).