• Catalog Number:

  E1A7057A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 362 (P-I-YP-D-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q99704

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  DOK1 Docking protein 1

• Specificity Sensitivity:

  p62Dok (Phospho-Tyr362) antibody detects endogenous levels of p62Dok only when phosphorylated at tyrosine 362.

• References:

  Zhou Songyang, et,al. (2001) J. Biol. Chem; 276: 2459-2465. Jean-Guy Némorin, et,al.(2000) J. Biol. Chem; 275: 14590. Yuji Yamanashi, et,al.(2000) Genes & Dev; 14: 11. Ibuki Kato, et,al.(2002) J. Immunol; 168: 629.

• All Sites:

  Human: Tyr362; Mouse: Tyr361; Rat: Tyr436

Western blot analysis of extracts from JK cells, using p62Dok (Ab-362) antibody (Line 1 and 2) and p62Dok (Phospho-Tyr362) antibody (Line 3 and 4).