• Catalog Number:

  E1A7056A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  Q9UD71

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B; PPR1B

• Specificity Sensitivity:

  DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.

• References:

  Ohta S., FEBS Lett. 242-31-35(1988). Kemmer D., BMC Genomics 7:48-48(2006). Martin J., Nature 432-988-994(2004).

• All Sites:

  Human: Thr34; Mouse: Thr34; Rat: Thr34

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using DARPP-32 (Phospho-Thr34) antibody. Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using DARPP-32 (Phospho-Thr34) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) antibody.