Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Dab1 around the phosphorylation site of tyrosine 232 (G-V-YP-D-V).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O75553
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Other Names:
disabled 1
Specificity Sensitivity:
Dab1 (Phospho-Tyr232) antibody detects endogenous levels of Dab1 only when phosphorylated at tyrosine 232.
References:
Lambert de Rouvroit C., Genomics 53:246-247(1998).
Gregory S.G., Nature 441:315-321(2006).
Ota T., Nat. Genet. 36:40-45(2004).
All Sites:
Human: Tyr232; Mouse: Tyr232; Rat: Tyr232
Western blot analysis of extracts from LOVO cells, using Dab1 (Phospho-Tyr232) antibody.