• Catalog Number:

  E1A7053A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R) (CILSRRPSYRKILN).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16220

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  CREB (phospho-Ser133) antibody detects endogenous levels of CREB only when phosphorylated at serine 133.

• References:

  Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55. Tan Y, et al. (1996) EMBO J; 15(17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (phospho-Ser133) antibody.

Western blot analysis of extracts from HeLa cells treated with UV, using CREB (phospho-Ser133) antibody.