• Catalog Number:

  E1A7049A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q14247

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  Amplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin

• Specificity Sensitivity:

  Cortactin (phospho-Tyr421) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 421.

• References:

  Du, Y. et al. (1998) Mol. Cell. Biol. 18, 5838-5851. Naisbitt, S. et al. (1999) Neuron 23, 569-582. Rodrigo, J. P. et al. (2000) Clin. Cancer Res. 6, 3177-3182. Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-6434.

• All Sites:

  Human: Tyr421; Mouse: Tyr421; Rat: Tyr383

Western blot analysis of extracts from Hela cells untreated or treated with H2O2, using Cortactin (Ab-421) antibody (Line 1 and 2) and Cortactin (phospho-Tyr421) antibody (Line 3 and 4).