Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 367 (R-A-SP-S-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17302
Applications:
WB: 1:500~1:3000 IHC: 1:50~1:100
ELISA: 1:20000
Alter Name:
Cx43; Gap junction 43 kDa heart protein
Specificity Sensitivity:
Connexin43 (Phospho-Ser367) antibody detects endogenous levels of Connexin43 only when phosphorylated at serine 367.
References:
Xiaoyong Bao, et al. (2004) Am J Physiol Cell Physiol; 286: C647-C654.
K Boengler, et al. (2006) Heart; 92: 1724-1727.
Satoshi Matsushita, et al. (2006) J. Histochem. Cytochem; 54: 343-353.
Holger K. Eltzschig, et al. (2006) Circ. Res; 99: 1100-1108.
All Sites:
Human: Ser367; Mouse: Ser367; Rat: Ser367
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Connexin43 (Phospho-Ser367) antibody.
Western blot analysis of extracts from K562 cells, using Connexin43 (Phospho-Ser367) antibody.