• Catalog Number:

  E1A7048A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 367 (R-A-SP-S-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17302

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  Cx43; Gap junction 43 kDa heart protein

• Specificity Sensitivity:

  Connexin43 (Phospho-Ser367) antibody detects endogenous levels of Connexin43 only when phosphorylated at serine 367.

• References:

  Xiaoyong Bao, et al. (2004) Am J Physiol Cell Physiol; 286: C647-C654. K Boengler, et al. (2006) Heart; 92: 1724-1727. Satoshi Matsushita, et al. (2006) J. Histochem. Cytochem; 54: 343-353. Holger K. Eltzschig, et al. (2006) Circ. Res; 99: 1100-1108.

• All Sites:

  Human: Ser367; Mouse: Ser367; Rat: Ser367

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Connexin43 (Phospho-Ser367) antibody.

Western blot analysis of extracts from K562 cells, using Connexin43 (Phospho-Ser367) antibody.