• Catalog Number:

  E1A7044A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68 (V-S-TP-Q-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  Chk2 (Phospho-Thr68) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 68.

• References:

  Gorgoulis VG, et al. (2005) Nature; 434(7035): 907-13. Falck J, et al. (2005) Nature; 434(7033): 605-11. Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81. Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.

Immunohistochemical analysis of paraffin- embedded lung carcinoma. Left: Using Chk2 (Phospho-Thr68) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.

Western blot analysis of the extracts from Jurkat cells untreated or treated with UV using Chk2 (Ab-68) Antibody and Chk2 (Phospho-Thr68) Antibody.