• Catalog Number:

  E1A7040A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  CHEK1, Chk1, kinase Chk1

• Specificity Sensitivity:

  Chk1 (phospho-Ser280) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.

• References:

  Puc et al. (2005) J Cancer Cell, 7, 2. Yuchi Hirose, et al. (2005) Cancer Research 65, 4861-4869, June 1. Vincent O'Brien, et al. (2006) Carcinogenesis 27(4):682-692.

• All Sites:

  Human: Ser280; Mouse: Ser280; Rat: Ser280

UV - + Western blot analysis of extracts from HT29 cells untreated or treated UV, using Chk1 (phospho-Ser280) antibody (Line 1 and 2).