• Catalog Number:

  E1A7038A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P30307

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CDC25M1; dual specificity phosphatase Cdc25C; EC 3.1.3.48; M-phase inducer phosphatase 3; MPI1B; MPIP3

• Specificity Sensitivity:

  CDC25C (Phospho-Ser216) antibody detects endogenous levels of CDC25C only when phosphorylated at serine 216.

• References:

  Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990). Bureik M., Int. J. Oncol. 17:1251-1258(2000). Livingston R.J., Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser216

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDC25C (Phospho-Ser216) antibody. Western blot analysis of extracts from HUVEC cells, treated with serum (20%, 30mins), using CDC25C (Phospho-Ser216) antibody.