• Catalog Number:

  E1A7036A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06493

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  p34 protein kinase; Cyclin-dependent kinase 1; CDK1

• Specificity Sensitivity:

  CDC2 (Phospho-Tyr15) antibody detects endogenous levels of CDC2 only when phosphorylated at tyrosine 15.

• References:

  Jeremy P. H. Chow, et al. (2003)J. Biol. Chem; 278: 40815-40828. NJ Wells, et al. (1999) J. Cell Sci; 112: 3361. P Jin, et al. (1996) J. Cell Biol; 134: 963. KM Pickham and DJ Donoghue (1994) Mol. Biol. Cell; 5: 587.

• All Sites:

  Human: Tyr15; Mouse: Tyr15; Rat:Tyr15

Western blot analysis of extracts from HepG2 cells, CDC2 (Ab-15) antibody (Line 1 and 2) and CDC2 (phospho-Tyr15) antibody (Line 3).