• Catalog Number:

  E1A7035A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (T-Y-TP-H-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06493

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  CDC2(Phospho-Thr161) Antibody detects endogenous levels of CDC2 only when phosphorylated at Threonine 161.

• References:

  Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6. Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53. Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56. Liu Y, et al.(2004)J Biol Chem; 279(6): 4507-14.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using CDC2(Phospho-Thr161) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis using CDC2 (Phospho-Thr161) Antibody: Line1: The extracts from COLO cells using preincubated with synthesized phosphopeptide; Line2: The extracts from COLO cells. Line3: The extracts from K562 cells.