• Catalog Number:

  E1A7034A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of Tyrosine 14(H-L-YP-T-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q03135

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  Caveolin-1(Phospho-Tyr14) Antibody detects endogenous levels of Caveolin-1 only when phosphorylated at Tyrosine 14.

• References:

  Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250. Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140. Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586. Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.

Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with H2O2 using Caveolin-1(Ab-14) Antibody and Caveolin-1(Phospho-Tyr14) Antibody: Line1: Using Caveolin-1(Ab-14) Antibody; Line2: Using Caveolin-1(Ab-14) Antibody preincubated with synthesized peptide; Line3 & Line4: Using Caveolin-1(Phospho-Tyr14) Antibody.