• Catalog Number:

  E1A7033A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CaMK2 around the phosphorylation site of threonine 286 (Q-E-TP-V-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UQM7/Q13557

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM-kinase II alpha chain; CaMK-II alpha subunit; CAMK2A; CaMKII-alpha; EC 2.7.11.17; KCC2A; kinase CaMK2-alpha

• Specificity Sensitivity:

  CaMK2 (Phospho-Thr286) antibody detects endogenous levels of CaMK2 only when phosphorylated at threonine 286.

• References:

  Li G.Y., Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. Nagase T., DNA Res. 6:63-70(1999). Krapivinsky G., Neuron 43:563-574(2004).

• All Sites:

  Human: Thr286/ Thr287; Mouse: Thr286/ Thr287; Rat: Thr286/ Thr287

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2 (Phospho-Thr286) antibody. Western blot analysis of extracts from K562 cells, using CaMK2 (Phospho-Thr286) antibody.

P-peptide - + Immunofluorescence analysis of COS-7 cells, using CaMK2 (Phospho-Thr286) antibody.