• Catalog Number:

  E1A7026A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 IP: Various Dilution ELISA: 1:20000

• Other Names:

  Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; BCL2

• Specificity Sensitivity:

  BCL-2 (Phospho-Thr56) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56.

• References:

  Tsujimoto Y., Proc. Natl. Acad. Sci. U.S.A. 83:5214-5218(1986). Eguchi Y., Nucleic Acids Res. 20:4187-4192(1992). Cleary M.L., Cell 47:19-28(1986).

• All Sites:

  Human: Thr56

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Phospho-Thr56) antibody. Western blot analysis of extracts from K562 cells, treated with H2O2 (100uM, 30mins), using BCL-2 (Phospho-Thr56) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using BCL-2 (Phospho-Thr56) antibody.