• Catalog Number:

  E1A7024A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from humanβ-Catenin around the phosphorylation site of Threonine 41/Serine 45 (A-T-TP-T-A-P-SP-L-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35222

• Applications:

  WB: 1:500~1:1000 ELISA: 1: 20000

• Specificity Sensitivity:

  β-Catenin (Phospho-Thr41/Ser45) Antibody detects endogenous levels of β-Catenin only when phosphorylated at Threonine 41/Serine 45.

• References:

  Provost E, et al. (2003)J Biol Chem 22; 278(34): 31781-9. Sakanaka C , et al. (2003)J Biochem (Tokyo); 132(5): 697-703. Liu C, et al. (2002) Cell; 108(6): 837-47. Provost E, et al. (2005) Oncogene; 24(16): 2667-76.

Western blot analysis of extracts from SW626 cell, using β-Catenin (Ab-41/45) Antibody and β-Catenin (Phospho-Thr41/Ser45) Antibody.