Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human β-Catenin around the phosphorylation site of serine 33 (L-D-SP-G-I).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35222
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Alter Name:
CATNB, CTNB1, CTNNB1, catenin, beta
Specificity Sensitivity:
β-Catenin (phospho-Ser33) antibody detects endogenous levels of β-Catenin only when phosphorylated at serine 33.
References:
Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379.
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200.
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195.
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764.
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952.
All Sites:
Human: Ser33; Mouse: Ser33; Rat: Ser33
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using β-Catenin (phospho-Ser33) antibody.