Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 136 (S-R-SP-A-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q92934
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at serine136.
References:
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.
Lee YI, et al. (2001). J Biol Chem.276 (20): 16969-16977.
Maiti D, et al. (2001). J Biol Chem.276 (1): 329-333.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho-Ser136) antibody.