• Catalog Number:

  E1A7017A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of Serine 245.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P18848

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  ATF4 (Phospho-Ser245) Antibody detects endogenous levels of ATF4 only when phosphorylated at Serine 245.

• References:

  Yang X, et al. (2004). Cell.117(3): 387-398.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ATF4 (Phospho-Ser245) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.