Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of Serine 245.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P18848
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Specificity Sensitivity:
ATF4 (Phospho-Ser245) Antibody detects endogenous levels of ATF4 only when phosphorylated at Serine 245.
References:
Yang X, et al. (2004). Cell.117(3): 387-398.
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ATF4 (Phospho-Ser245) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.