• Catalog Number:

  E1A7011A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q99683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  Apoptosis signal- regulating kinase 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen-activated protein kinase kinase kinase 5

• Specificity Sensitivity:

  ASK1 (Phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.

• References:

  Wang X.S., J. Biol. Chem. 271:31607-31611(1996). Ichijo H., Science 275:90-94(1997). Mungall A.J., Nature 425:805-811(2003).

• All Sites:

  Human: Ser966; Mouse: Ser973

Western blot analysis of extracts from 293 cells, treated with TNF (20ng/ml, 15mins) and calyculin A (50nM, 15mins), using ASK1 (Phospho-Ser966) antibody.