Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Androgen Receptor around the phosphorylation site of serine 650 (T-T-SP-P-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Androgen Receptor (phospho-Ser650) antibody detects endogenous levels of Androgen Receptor only when phosphorylated at serine 650.
References:
Christin E. Petre-Draviam, et al. (2003) Cancer Res. 63: 4903-4913.
Sydney A. Shain, et al. (1983) Cancer Res. 43: 3691-3695.
Bekir Cinar, et al. (2005) Cancer Res. 65: 2547-2553.
Soo-Yeon Park, et al. (2006) Cancer Res. 66: 5121-5129.
All Sites:
Human: Ser650; Mouse: Ser650
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using Androgen Receptor (phospho-Ser650) antibody.